The Science of Dank

Learn about the groundbreaking discovery of Cannasulfur Compounds.

We’ve all smelled it – that distinctive, gassy, or skunk-like scent of cannabis. But what exactly is that smell? Researchers at Abstrax Tech, in collaboration with some of the top cultivators in the industry and analytical experts, have now answered this question — and the answer may surprise you. 

Cannabis produces arguably some of the most unique and potent aromas of any plant. However, figuring out what makes this plant smell the way it does is no easy feat. A survey of cannabis literature makes no correlation toward the "skunk-like" or "gassy" aroma that it’s so infamous for, highlighting the difficulty in determining the origins of this scent.

This has now changed, however, as research published in November 2021 by Abstrax Tech now unlocks the mysteries of this part of the plant, revealing an entirely new class of compounds. 

Read on and learn how we discovered cannasulfur compounds (CSCs), their aromatic and possible therapeutic properties, and future work needed to further understand these unique compounds. 

Filling in the Cannabis Aroma Gaps

In recent years, much focus within the cannabis industry has shifted toward terpenes, whether from a research or marketing standpoint. These compounds are the major components of the aroma of cannabis by weight and are regularly tested for by cannabis analytical labs. They include ß-myrcene, α- and ß-pinene, linalool, ß-caryophyllene, and myriad others. 

These compounds are being heralded as the true driving force between different psychoactive effects in cannabis in conjunction with THC, colloquially known as the Entourage Effect. They are also considered convenient biomarkers for establishing genetic classes between different phenotypes or chemotypes within the cannabis phase space. 

However, these compounds do not create the unmistakable "gassy" or "skunky" scent many high-quality cultivars are prized for. In fact, we argue that until now, what some scents many consumers and experts say is due to terpenes, we now posit is incorrect, and is based on an incomplete picture of the aroma of cannabis. 

Yes, terpenes indeed contribute to the bouquet of cannabis’ aroma, but we now know that some of the most coveted scents are not from these commonly known terpenes at all.

Research conducted by Abstrax Tech between 2019 and 2021 has identified an entirely new class of previously unknown compounds that are the true origins of the "gas, skunk, and dank". We refer to this new class of compounds as "cannasulfur compounds (CSCs)", due to the presence of sulfur in each of them (Figure 1). 

Figure 1

Figure 1. Newly discovered cannasulfur compounds (CSCs) in cannabis. Compounds CSC3 - CSC7 contribute to the skunky or gassy aroma of cannabis.

Each of these compounds contains the prenyl functional group bonded to a sulfur atom. Some are thiols, others are sulfides, and yet others are disulfides. These compounds all have extremely low odor thresholds, as well as have moderate-to-high volatilities – the perfect recipe to be detectable to the human nose over large distances in low quantities. 

Finding Cannasulfur Compounds in Cannabis

Abstrax Tech has been intensively researching cannabis since 2019, aimed at understanding the nuances of the many aromas this plant can produce. The research and development team first teamed up with Markes International and SepSolve Analytical, two instrument manufacturers that specialize in multidimensional gas chromatography, to develop the ultimate analytical tool for decoding the scent of cannabis. 

With the experts on the instrumentation side, a 2-dimensional gas chromatography instrument with three detectors operating simultaneously was constructed. Each detector was chosen to serve a specific function: Mass spectrometry was used for chemical identification, flame ionization for quantitation, and sulfur chemiluminescence for detection of sulfur-containing species. 

These three detectors, in conjunction with the enhanced separatory power of 2-dimensional gas chromatography, gave the research team the necessary firepower to fully understand the complex aroma of cannabis.

2-Dimensional Gas Chromatography: A Crash Course

When you smell your precious cannabis flowers, what exactly are you smelling? Is it a single chemical compound? A few? The answer is hundreds. But how is this figured out? That is where gas chromatography (GC) comes into play. 

Gas chromatography is the technique of taking a sample of a "smell" – i.e., a combination of volatile chemical compounds in the gas phase – and passing it through a thin, porous (i.e., hollow) tube called a column. 

Figure 2

Figure 2. Simplified schematic of how a traditional 1-dimensional gas chromatography instrument operates.

A sample of cannabis flower is first put into an air-tight container. The compounds that make it smell the way it does will naturally enter the empty space of the container. A needle will then go into the vial and draw up a sample of these compounds, much like drawing blood with a needle. In this case, we are drawing up the compounds that give the flower its scent. The needle then takes those compounds and injects them into the porous, hollow column of the GC.

At the beginning, where they are first injected, the gaseous compounds are all grouped together, just like they would be when smelling a flower. As the gases pass through the column, certain compounds will move more slowly than others. Larger compounds will travel more slowly than smaller ones due to their larger size and get "stuck" to the sides of the column. 

An example of this is α-pinene and ß-caryophyllene: α-pinene is a monoterpene with fewer atoms in its structure, and thus is physically smaller than ß-caryophyllene. It will therefore pass through the column more quickly than ß-caryophyllene. 

Columns can be upwards of 30 meters long, which gives the different compounds enough time and space to separate (Figure 1) into discrete packets. By the time the various compounds arrive at the end of the column, they are (hopefully) fully separated into their own packets. These packets are then passed from the column to a detector, which can tell the user what each packet of compounds are. 

From here, the user can determine how many and the identity of each packet of compounds, and determine the chemical composition of the aroma of the cannabis flower.

Figure 3

Figure 3. Simplified schematic of how 2-dimensional gas chromatography operates.

But what happens when even after the 30 meters of separating in the column, the packets are not completely separated? This is where having a second column, capable of separating these compounds further based on other chemical attributes, such as polarity, comes in. 

In 2-dimensional gas chromatography (GC x GC), the compounds travel along a first column just like in traditional GC and separate based on the size of the compounds. However, as they do this, they are also passed through a second column that separates the compounds based on their polarities (Figure 3). The detector can then detect these compounds based on size and polarity, allowing all the packets of compounds to be even more efficiently separated from one another.

As Abstrax has detected upwards of 400 compounds in certain cultivars, it becomes obvious why having two degrees of separation in GC x GC is more powerful than one degree in traditional GC.

Detection and Quantitation of Compounds Using Multiple Detectors

Building out analytical equipment is one thing, but answering scientific questions is another. At Abstrax Tech, we aim to understand every nuance of the aroma of cannabis. The biggest question our team and many others in the industry have had for years is, "What is the gas?" 

In 2019, that search began in earnest. Hundreds of cannabis flower samples were procured from some of the most prized growers in California and analyzed. 

These include Mario Guzman of Sherbinskis – famed for his creation of the Gelato line, Pink Panties, and Sunset Sherbet – all now mainstays of the cannabis lexicon – and Josh Del Rosso, the originator of OG Kush. This legendary cultivar is the “War Admiral” of cannabis – the broodmare that has been crossed to produce many more highly desired cultivars, including some bred by Guzman. Both Guzman and Del Rosso, along with the team at Abstrax Tech, wanted to answer this longstanding question once and for all.

Table 1

Detection and Quantitation of Compounds Using Multiple Detectors Answering the question, "What compound(s) produce the gassy and skunky scent in cannabis?"

After collecting data on hundreds of cultivars, differences in the data were analyzed meticulously, looking for trends between them. Sativas, Hybrids, Indicas, even hemp, were used to home in on one or more compounds that may be present in all the cultivars that were considered to be gassy or skunky. One by one, different compounds were eliminated as the potential source of these compounds.

However, doing this for hundreds of compounds would have been a monumental feat. Luckily, that is where chemical intuition comes into play: The scientists at Abstrax hypothesized that the potent, gassy, scent of cannabis may contain sulfur due to the fact that many other highly potent fruits or plants contain compounds with sulfur. Durian, hops, onion, and garlic each have potent aromas, and each contain compounds with sulfur in their chemical structures. 

This hypothesis allowed the team to focus their efforts on compounds that appeared in the sulfur chemiluminescence data. Immediately, trends were formed between cultivars described as skunky or gassy and compounds that contained sulfur. 

Figure 4 shows the SCD data for Bacio Gelato, a highly potent (both in effects and aroma) cannabis cultivar, and Black Jack, a cultivar that has a minimal gassy or skunk-like scent. This data provided evidence that pungent cannabis cultivars such as Bacio Gelato contain fundamentally different compounds than those found in those that do not have this scent, such as Black Jack.

Want more details on this gassy cultivar? Get all the details in Bacio Gelato: The Gas Giant of Exotic Cannabis.

Once it was established that there is high probability that these compounds could be contributing to the gas, their chemical identities needed to be determined. Although one was found in chemical databases, 3-methyl-2-butene-thiol (CSC3), also known as prenyl thiol, the others were not. This meant that these other compounds were potentially completely new compounds, or at least rarely discussed in any scientific medium. 

The chemists at Abstrax worked through the mass spectral data to discover that each of these compounds contains the prenyl functional group. Each compound was then chemically synthesized and measured using GC x GC, which confirmed their hypothesized chemical identities. Aroma tests of these CSCs, along with terpenes, confirmed that each of these compounds contributes to the gassy, skunky scent of cannabis, answering the longstanding question of what the chemical origins of these scents were.

Figure 4

Figure 4. Data showing many more CSCs in a gassy strain than a non-gassy strain. Bacio Gelato (C1), has numerous CSCs present, whereas Black Jack (C13) does not, indicating that the potent, gassy aroma of Bacio is due to these CSCs.

How Are CSCs Produced During Plant Growth?

Once we discovered these compounds exist in cannabis, we asked the question, “when are CSCs produced by cannabis during growth?” As the gas is a crucial scent generated by this plant that consumers highly desire, we wanted to answer this question. 

We conducted a study by monitoring the amounts of each CSC as a function of week during plant growth. What we found was truly fascinating: During the early weeks of flowering, we did not measure any appreciable amount of any CSC. However, toward the end of the flower stage, we observed a sharp increase in each, as shown in Figure 5. During the final week of flowering, week 10, each increased substantially. We then observed a huge increase of each CSC at the end of curing (Week 11 in Figure 5). 

Figure 5

Figure 5. Change in CSC concentrations as a function of cannabis plant growth. These data show that CSCs increase substantially during the final weeks of flowering into curing.

This indicates that as the plant is dried and cured, CSCs increase rapidly. This represents the gassiest, most potent example of the flower grown, as after just 10 days, the concentration had already dropped significantly. Our results show that not only do these compounds get produced rapidly towards the end of the grow cycle, but they also decrease rapidly after harvest. 

Want to learn more about gassy cannabis? Read ALL about it in Top 10 Gassiest Cultivars We’ve Measured (So Far)

We hope these results will help growers consider that time is of the essence when producing and packaging their products. Further, proper packaging will likely also help in the retention of these compounds, thereby allowing their products to retain their optimal scent for as long as possible.

Figure 6

Figure 6. GC x GC chromatograms showing the presence of important CSCs in butane hash oil (BHO) samples. These data suggest that proper extraction techniques can retain these key compounds.

Can CSCs be found in Cannabis extracts?

One question that may arise from consumers of cannabis extracts is whether these very volatile compounds are preserved in an extract such as butane hash oil (BHO). The answer we found is yes, if processed correctly. 

At Abstrax Labs, our sister company that produces premium cannabis extracts, we tested whether these compounds can be preserved. Using state-of-the-art technologies, including cryogenic extraction processes and minimal heat post-processing, we found that indeed, these compounds can be preserved in high concentrations. 

In fact, we found that certain CSCs typically not seen in high amounts in flower after the curing process, such as 3-methyl-2-butenyl acetothioate (CSC5), can be preserved significantly during the extraction process. Figure 6 shows examples of three different samples containing CSCs, some of which would not be retained without the proper manufacturing process. These results prove that, yes, cannabis extracts can indeed contain these important compounds, but must be processed correctly to do so.

Potential Medicinal Properties of CSCs

Discovering CSCs in cannabis opens the door for many different questions to be posited and answered. A major one that immediately comes to mind is whether these compounds may provide cannabis with more medicinal qualities than initially thought. 

During the initial discovery of CSCs, researchers at Abstrax Tech soon realized that these compounds, although relatively simple in structure, are strikingly similar to those found in garlic, as shown in Figure 7. The only difference between CSCs in cannabis and compounds in garlic is the extra methyl groups in the prenylated compounds.

Figure 7

Figure 7. CSCs in cannabis and garlic. Chemical similarities suggest similar biological activity maybe present, however further studies must confirm this.

These compounds in garlic are some of the most important compounds in terms of health benefits in our diets. Thus, it begs the question whether CSCs in cannabis may likewise possess beneficial health benefits, although further research is needed to confirm. 

Lastly, we note that although the prenyl functional group is ubiquitous in protein chemistry and biosynthesis of secondary metabolites, cannabis appears to contain an unusual amount (Figure 8). 

For instance, cannflavins, which are prenylated flavonoids that are unique to cannabis, each contain the prenyl functional group. Likewise, many important cannabinoids, including CBGA and CBCA, contain this group as well. With CSCs also containing this group, it opens the question as to why cannabis produces so many secondary metabolites with this structure. This and other open-ended questions are now possible to answer with the discovered compounds.

Figure 8

Figure 8. Prenylated secondary metabolites in cannabis. The reason for why cannabis produces so many compounds with this functional group should be further explored.

Abstrax Tech | Pioneering Cannabis Aroma Science

The discovery of CSCs in cannabis started as a mission to determine the quintessential gassy and skunky scent of cannabis. However, understanding these aroma properties of cannabis may be just the tip of the iceberg. 

The revelation that an entirely new class of compounds with similar functionality to those in other plants, such as garlic, opens the door to further understanding if they possess any medicinal properties. 

We lastly note that although the structures of these compounds were elucidated, other CSCs most likely exist but have yet to be fully identified. Future studies will hopefully reveal even more interesting compounds in this plant.

Identification of a New Family of Prenylated Volatile Sulfur Compounds in Cannabis Revealed by Comprehensive Two-Dimensional Gas Chromatography. Iain W. H. Oswald, Marcos A. Ojeda, Ryan J. Pobanz, Kevin A. Koby, Anthony J. Buchanan, Josh Del Rosso, Mario A. Guzman, and Thomas J. Martin.

ACS Omega 2021 6 (47), 31667-31676DOI: 10.1021/acsomega.1c04196

Experience Prenylated Cannasulfur Compounds

SHOP Bacio Gelato

Out of the tens of thousands of cultivars we’ve analyzed, Bacio Gelato reigns supreme as the gassiest strain ever measured. Created in partnership with Mario Guzman of Sherbinskis, this is the world’s only authentic and official Gelato terpene profile.

SHOP King Louie XIII

Heavy is the head that wears the crown. Indeed, this monarch carries the traditional OG impact concentrated in a pleasantly cranial direction. This profile is just straight gas. If you want to get loud, look no further. The king is here.

SHOP GMO

As the first to benefit from our breakthrough discovery, GMO is kicking off the next era of botanical aromatics. Flavorants Skatole and Indole multiply its notoriously savory and funky notes for a loud, gassy, and undeniably pungent finish.

SHOP Acapulco Gold

Acapulco Gold is far out, man. With its rich combo of gilded honey, bright citrus, and that splash of gas, this landrace cultivar perfectly captures the cool gulf breezes and blazing Mexican sun of the 60s.

SHOP Super Sour Diesel

As a cross between Super Silver Haze and Sour Diesel, it should come as no surprise that Super Sour Diesel is fuel-forward with pungent notes of pine, earth, and citrus. Prepare for a sauce that’s unforgettably potent.

SHOP Dynasty OG

Dynasty OG has inherited all of the authoritative dankness of its OG Kush lineage. Regal elements of pine, citrus, and earth merge with loud gassy aromas to inspire loyalty, relaxation, and respect for the OG.

SHOP Munson

A celebration of dank, skunky, old-school hash tradition, Munson combines Northern Lights and The Skunk for a rich, pungent, and bracing experience that will make long-haulers feel right at home.

SHOP GG4

Respected, often feared, and usually sold out - Gorilla Glue #4 is potent in more ways than one. The aroma is mildly floral but mainly earthy with tangible notes of wood, spice, and skunk. Just don’t try to fight the euphoric couch lock.

SHOP RNTZ OG

If you're a fan of Pixy Stix, you'll appreciate the sweet and sour candy notes of this blend. It'll hit you with shockingly bright citrus before it slams into a spicy finishing move. It's fresh, mouthwatering, and always leaves you wanting more.